Francis Coquelin started at centre-back as part of a back three during Arsenal's loss against Manchester City.
Premier League leaders Manchester City are expected to revisit their interest either in January or at the end of the season.
Kolasinac, who arrived on a free transfer from Schalke this summer, has quickly grown into an influential figure at the Emirates.
Arsene Wenger has made it clear that Alexis Sanchez will not be sold to Manchester City during the January transfer window.
Arsenal are massive underdogs going into the fixture, trailing the league leaders by nine points, with 10 games played thus far in the campaign.
Arsene Wenger has confirmed that Sunday's trip to Manchester City will come too soon for David Ospina, Shkodran Mustafi and Danny Welbeck.
The Gunners have not won the title since 2004 when Arsene Wenger could call on the likes of Patrick Viera, Ashley Cole and Thierry Henry, among others.
Wilshere was not included in the squad for the England friendlies next week despite Wenger stating that he believes the midfielder is ready to get back into the international fold.
Arsene Wenger happy with his selection policy after Arsenal's second-string stutter during dull Europa League draw against Red Star Belgrade.
The Gunners will play their fourth group game of the campaign against Red Star Belgrade in the Europa League, having won all three games thus far.
Thomas Lemar had attracted interest from Arsenal and Liverpool in the summer transfer window, with both clubs making an approach for the Frenchman.
Arsene Wenger is certain that Wilshere is ready to return to the Three Lions set up after proving his fitness with Arsenal this season.
Arsene Wenger suggests the Gunners will not make a move to sign Thomas Lemar from Monaco during the January transfer window.
Teenage Arsenal winger Reiss Nelson has revealed how he spent a month with Tottenham before eventually joining the Gunners' academy as a child.
Arsene Wenger says forgotten defender Mathieu Debuchy has impressed him as a centre-half, suggesting the Frenchman could have a future in that position at Arsenal.
Sanchez, 28, came close to joining City during the summer transfer window, but the move broke down on deadline day.
Arsenal will begin talks with 18-year-old Eddie Nketiah over a new contract after his Carabao Cup heroics against Norwich City last week.
The Citizens have been in brilliant form this season, winning nine of the 10 games thus far, while drawing the other.
Nketiah proved his worth coming on as a second half substitute, scoring two goals against Norwich City to lead the north London club to the fifth round of the Carabao Cup.
The Gunners last won the title in 2003-04 when they went the entire season unbeaten but recent years have seen the competition pass Wenger by.
Arsene Wenger has revealed that he is unlikely to make too many changes to his Premier League team and it will be almost the same as the one that beat Everton.
Walcott has seen just 47 minutes of Premier League action this season and is far down the pecking order in north London.